Hyaluronic Acid Hydrogel Cell Culture

Hyaluronic acid hydrogel integrated with mesenchymal stem cell secretome to treat endometrial injury in a rat model of asherman s syndrome feiran liu department of gynecology and obstetrics beijing hospital national center of gerontology beijing 100730 china.

Hyaluronic acid hydrogel cell culture.

These results are useful for designing conditions for using gelatin hyaluronic acid composite hydrogels. Cell culture and encapsulation. Hyaluronic acid ˌ h aɪ. Their main component is one of the major constituents of the extracellular matrix ecm hyaluronic acid ha.

Similarly sn hydrogels were prepared by mixing ha hydrazine and ha ald or ha bld stock solutions. Conjugate base hyaluronate also called hyaluronan is an anionic nonsulfated glycosaminoglycan distributed widely throughout connective epithelial and neural tissues it is unique among glycosaminoglycans in that it is nonsulfated forms in the plasma membrane instead of the golgi apparatus and can be very large. The combination of cells and hydrogel has led to enhanced cardiac repair in both large and small animal models of mi compared to injection of cells alone. Of the synthetic and naturally derived biomaterials that have been examined hyaluronic acid ha based hydrogels have attracted much interest.

The material can be chemically and mechanically tuned and provides a fully defined and scalable method for stem cell expansion. Here we report that culture of human ns pcs in 3d hyaluronic acid ha rich biomaterial microenvironments increased differentiation toward oligodendrocytes and neurons over 2d cultures on laminin coated glass. A hydrogel was formed within 5 min after mixing with pipette for five times. Ha is the simplest glycosaminoglycan a negatively charged linear non sulfated polysaccharide that provides compression strength lubrication and hydration within the ecm 1.

Hystem is a semi synthetic 3d hydrogel platform that allows cell growth in a 3d environment. This work reports a thermoresponsive hyaluronic acid and poly n isopropylacrylamide based hydrogel that supports human pluripotent stem cell culture from single cells. Moreover ns pcs in 3d culture exhibited a significant reduction in differentiation into reactive astrocytes.