Human Bronchial Epithelial Cell Line

Aspirate the supernatant from the tube without disturbing the cell pellet.

Human bronchial epithelial cell line.

16hbe14o cells have a cobblestone appearance in culture form tight junctions and display. Investigation of this viral infection in the human bronchial epithelial cell line 16hbe suggests that sars cov 2 can enter these cells through interaction between its membrane localized s protein with the angiotensin converting enzyme 2 molecule on the host cell membrane. Primary human bronchial epithelial cells hbepc are isolated from the surface epithelium of human bronchi and stain positive for cytokeratin. Flick the tip of the conical tube with your finger to loosen the cell pellet.

These cells are ideally suited to support the study of many fields including inflammation cancer gene regulation cell matrix interactions and also have application in toxicology testing and drug screening development. 16hbe14o is a human bronchial epithelial cell line isolated from a 1 year old male heart lung patient and immortalized with the origin of replication defective sv40 plasmid psvori. Calu 3 cells human bronchial epithelial cell line are originally derived from the subbronchial epithelium of a lung adenocarcinoma atcc manassas va and have been used as surrogate nasal epithelium due to their similar biophysical properties such as forming tight monolayers and also secreting mucus when cultured in an air liquid. Further observation indicates distinct viral replication with a dynamic.

Resuspend the cells in 2 ml of bronchial tracheal epithelial growth medium by gently pipetting the cells to break up the clumps. The cells are cryopreserved at the second passage to ensure the highest viability and plating efficiency. The cell line retains characteristic features of normal differentiated bronchial epithelial cells including a cobblestone morphology cytokeratin expression the. Atcc primary cell solutions offers epithelial cells isolated from human breast bronchi trachea and small airways cornea prostate and kidneys.

The 16hbe14o cell line was originally developed from human bronchial epithelium to study the cystic fibrosis transmembrane conductance cftr regulator cozens et al 1994 and retains many features of differentiated bronchial epithelial cells. The respiratory epithelia are responsible for the lubrication of the lungs the maintenance of humidity and the cleaning of the respiratory tract. 16hbe14o human bronchial epithelial cell line 16hbe14o human bronchial epithelial cell line is widely used to model barrier function of the airway epithelium and to study respiratory ion transport as well as the function of cftr.